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KMID : 0362919940120010027
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Journal of the Korean Society for Therapeutic Radiology and Oncology
1994 Volume.12 No. 1 p.27 ~ p.31
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In Vitro Intrinsic Radiosensitivity Of Human Squamous Cell Carcinoma in Primary Culture
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Choi Eun-Kyung
Yang Gwang-Mo
Yi Byong-Yong
Chang Hye-Sook
Kim Sang-Yoon
Nam Joo-Hyun
Yu Eun-Sil
Lee In-Chul
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Abstract
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There are a number of reports suggesting that there may be a correlation between the clinical response to radiotherapy in various tumors and the clonogenic survival of cell lines derived from these tumor following exposure to 2 Gy (SF2). Authors conducted this study to determine SF2 for cells in primary culture from surgical specimens.
The tumor tissues with squamous cell carcinoma of uterine cervix and head and neck were obtained. The tumor tissues were disaggregated to single cells by incubating with collagenase type Ⅳ for 2 hours with constant stirring. Single cell suspensions were inoculated in four 24-well plates precoated with cell adhesive matrix. After 24 hours of incubation at 37℃, rows of four wells were then irradiated, consisting of control set and five other sets each receiving doses of 1,2,3,4, and 6 Gy. After incubation for a total of 13 days, the cultures were stained with crystal violet determine whether cell growth was of epithelial origin, immunocytochemical staining with a mixture of cytokeratin and epithelial monoclonal antibodies were performed on cell cultures.
During the period of this study, we received 5 squamous cell carcinoma specimens of head and neck and 20 of uterine cervical carcinoma. Of these, 15 yielded enough cells for radiosensitivity testing. This resulted an overall success rate of 60%. The mean SF2 value for 15 tumors was 0.55±0.17 ranging from 0.20 to 0.79.
These results indicate that there is a broad range of sensitivities to radiation in same histologic type. So with a large patient population, we plan to determine whether a different SF2 value is associated with tumors that are controlled with radiotherapy than those that are not.
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KEYWORD
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Survival at 2Gy, Primary culture, Squamous cell carcinoma, Head and neck cancer, Cervix cancer
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